Excessive secretion of TGF-β by cancer cells makes them irresponsive to growth inhibitory activity of TGF-β. Small interfering RNA (siRNA) mediated knockdown of TGF-β can restore the TGF-β tumor suppressing response in cancer cells and promotes apoptosis. For this study, exosomes isolated from HEK293T cells were loaded with siRNA targeting TGF-β1 and delivered to the cancer cells. Transfection of si-TGF-β1 packaged exosomes in HeLa cells significantly reduced TGF-β expression and induced apoptosis. Thus, exosomes can serve as potential candidates for siRNA drug delivery. Encapsulating siRNA in exosomes not only protects them from degradation by nucleases but also facilitates their uptake by the cells. The ultimate objective of this research is to achieve personalized medicine approach against cancer, where tumor tissue taken from the patient will be treated with siRNA drug loaded exosomes obtained from the serums of the same patient.