This presentation will highlight the development of advanced biomaterials and pioneering bioengineering techniques to grow engineered tissues for regeneration and disease modelling. By incorporating micro-nano features into biomaterial, the growth and functional markers of human corneal endothelial cell were enhanced, resulting in a functional and transplantable corneal endothelium graft to treat corneal blindness. In another application, using hydrogel biomaterial that enable light-triggered cell signaling, stem cells were differentiated spatio-temporally into bile duct cells. Moreover, a viscoelastic, tissue-mimicking hydrogel was developed for the 3D culture of liver bile duct organoids for disease modelling. Finally, I will discuss a range of novel hydrogels that are injectable, bio adhesive, and stimuli- responsive, improving graft-host integration and enabling controlled biotherapeutic delivery. These innovative biomaterials offer extensive tunability, and provide a versatile platform to manipulate cell behavior, modulate the immune response, and achieve regeneration.