Event date:
Apr 23 2021 10:30 am

Biochip with Multi-planar Electrodes Geometry for Differentiation of Non-Spherical Bioparticles in a Microchannel

Supervisor
Dr. Nauman Zafar Butt
Student
Amina Farooq
Venue
Zoom Meetings (Online)
Event
PhD Research Seminar
Abstract
A biosensor capable of differentiating cells or other microparticles based on morphology finds significant biomedical applications. Examples may include morphological determination in the cellular division process, differentiation of bacterial cells, and cellular morphological variation in inflammation and cancer etc. Here, we present a novel integrated multi-planar microelectrodes geometry design that can distinguish a non-spherical individual particle flowing along a microchannel based on its electrical signature. We simulated multi-planar electrodes design in COMSOL Multiphysics and have shown that the changes in electrical field intensity corresponding to multiple particle morphologies can be distinguished. Our initial investigation has shown that top-bottom electrodes configuration produces significantly enhanced signal strength for a spherical particle as compared to co-planar configuration. Next, we integrated the co-planar and top-bottom configurations to develop a multi-planar microelectrode design capable of electrical impedance measurement at different spatial planes inside a microchannel by collecting multiple output signatures. We tested our integrated multi-planar electrode design with particles of different elliptical morphologies by gradually changing spherical particle dimensions to the non-spherical. The computed electrical signal ratio of non-spherical to spherical particle shows a very good correlation to predict the particle morphology. The biochip sensitivity is also found be independent of orientation of the particle flowing in the microchannel. Our integrated design will help develop the technology that will allow the morphological analysis of various bioparticles in microfluidic channel in the future.

Meeting Link: https://lums-edu-pk.zoom.us/j/91014515295?pwd=WXR1U1RySlZOSkI0Rmhud0FkMWVCUT09

Meeting ID: 910 1451 5295
Passcode: 287095