Dengue virus (DENV) infection has become a severe public health concern globally, especially in tropical and subtropical countries. Dengue fever is a mosquito-borne viral infectious disease with a wide range of clinical manifestations, from a mild cold-like illness to the serious hemorrhagic dengue fever (DHF) and dengue shock syndrome (DSS). The causative agents are dengue virus serotype 1,2,3 and 4 (DENV1,2,3,4). The envelope protein (E) is a major component of the DENV virion surface, and it plays a key role during the viral entry process, constituting an attractive target for the development of antiviral drugs. However, currently, there is neither an approved antiviral drug nor an effective vaccine available to fight the disease. In this study, we aim to develop pseudovirus based cellular infection assay to identify viral cellular entry inhibitors as potential antiviral agents. To achieve this, we will use a combination of molecular biology and cell culture techniques.