Zetasizer (Nanoparticles Size/Zetapotential Analyzer)


Make and Model:

Malvern Zetasizer Nano ZSP


The Zetasizer Nano ZSP is used for the measurement of size, electrophoretic mobility of proteins, zeta potential of nanoparticles and surfaces, and optionally the microrheology of protein and polymer solutions. The exceptional performance also enables the measurement of the concentration, molecular weight and second virial coefficient, A2, of macromolecules and kD, the DLS interaction parameter. The system can also be used in a flow configuration to operate as a size detector for Size Exclusion Chromatography (SEC) or Field Flow Fractionation (FFF).


Particle and molecule size

·   Measurement range: 0.3nm – 10.0 microns (diameter)

·   Measurement principle: Dynamic Light Scattering

·   Minimum sample volume:12µL

Zeta potential and protein mobility

·   Measurement range:3.8nm – 100 microns (diameter)

·   Measurement principle: Electrophoretic Light Scattering

·   Minimum sample volume:150µL (20µL using diffusion barrier method)

Molecular weight

·   Measurement range: 980Da – 20M Da

·   Measurement principle: Static Light Scattering using Debye plot

·   Minimum sample volume:12µL (3-5 sample concentrations required)


Dr. Irshad Hussain (ihussain@lums.edu.pk)