Molecular Cloning and Characterisation of Two Clinically Important Enzymes, Malate Dehydrogenase and Aspartate Aminotransferase, of Thermophilic and Hyperthermophilic Origin
Dr. Ghazaleh Gharib
Date: April 19, 2017
Time: 3:30 PM – 4:30 PM
Venue: Smart Room, Syed Babar Ali School of Science and Engineering (SBASSE) 5th Floor
Host: Dr. Amir Faisal
Pcal-1699, a malate dehydrogenase (MDH) from hypethermophilic archaeon, Pyrobaculum calidifontis was cloned expressed and characterized and structural studies proved to be the most thermostable MDH reported to date. Two enzymes of aspartate aminotransferase (AST) and (MDH) from thermophilic Geobacillus SBS-4S isolated from Pakistan’s northern region were also cloned and expressed and their sequences submitted to NCBI. Biophysical and biochemical structural studies of these thermophilic enzymes were compared with their archaeon counterparts. Results demonstrated that thermostable MDH and AST with their unique properties could be potential sources for clinical and industrial applications for designing immunoassay for detection of various antibodies.
Dr. Gharib received her B.Sc. honours in subject of General Biology from University of Mashhad, Iran in 2004. She completed her MSc in Biotechnology from Institute of Biochemistry and Biotechnology University of the Punjab in 2008 and the same year was successfully enrolled in a MPhil leading to PhD in Biotechnology precisely enzymology from School of Biological Sciences - University of the Punjab under HEC scholarship. During last year of her PhD Dr. Gharib served as the visiting faculty at "De' Montmorency College of Dentistry Lahore” to teach undergraduate students biochemistry and was awarded with an outstanding teaching certificate for her unique teaching method. As a senior researcher, Dr. Gharib worked on project entitled "Study of Multi-Drug Resistant of Over 1000 Outdoor Pakistani Patients" as a collaborative project between University of Helsinki in Finland and School of Biological Sciences - University of the Punjab in late 2016. Currently, she is a visiting faculty member of biochemistry subject at University of Central Punjab, (UCP), Lahore.